Homework Solution: the column of chromatogrphy colour was pink yellow orange of experiment [Co(en)3 ]Cl3.3H2O…

    raph Renn 21/08/ 170821 15271 Al(acac3 in CDCI coCl IC2280so2.-1 H NMR ppa 10 Al (acac in CDCs (3 ッ. NMR 20 20D
    the column of chromatogrphy colour was pink yellow orange of experiment [Co(en)3 ]Cl3.3H2O Tris(ethylenediamine)cobalt(III). Thats the procedure of the experiment Mix up a slurry of CM-Sephadex C-25 (5 g) in water. Partly fill the column with water and add the slurry of Sephadex. The column should be about two-thirds full, with no occluded air bubbles. Allow the Sephadex to settle. Wash the column through with deionised water. Do not allow the column to drain free of solvent. Run solvent from the column until the meniscus is just level with the top of the column. Use the sample provided for your chromatography. Add this gently to the top of the column to a depth of approximately 1 cm, being careful not to disturb the gel. Turn the column on to allow the meniscus to drop to the top of the Sephadex. Carefully add approximately 1 cm3 of water to the top. Drain water from the bottom, until the meniscus drops as far as the top of the Sephadex. Repeat the addition and removal of water. Repeat this process with 0.5M NaCl. Gently fill up the column with 0.5M NaCl and elute the solution at a slow flow rate. Note the separation of the components as the column progresses. Collect the eluate in 10 cm3 portions and note the colour of each fraction. Alternatively, students may examine each fraction spectroscopically. Cells of 1 cm path length may be used although if the solutions are very weak longer path length cells may be necessary. Once completed, elute the column with concentrated NaCl solution to remove any traces of product. Empty the CM-Sephadex into the labelled collection beaker.
    raph Renn 21/08/ 170821 15271 Al(acac3 in CDCI coCl IC2280so2.-1 H NMR ppa 10 Al (acac in CDCs (3 ッ. NMR 20 20D

    Expert Answer

    raph Renn 21/08/ 170821 15271 Al(acac3 in CDCI coCl IC2280so2.-1 H NMR ppa 10 Al (acac in CDCs (3 ッ. NMR 20 20D

    the shaft of chromatogrphy colour was pink yellow orange

    of exemplification [Co(en)3 ]Cl3.3H2O Tris(ethylenediamine)cobalt(III).

    Thats the order of the exemplification

    Mix up a slurry of CM-Sephadex C-25 (5 g) in breathe-into. Partly swell the shaft with breathe-into and supplement the slurry of Sephadex. The shaft should be environing two-thirds liberal, with no occluded intelligence bubbles. Authorize the Sephadex to arrange. Wash the shaft through with deionised breathe-into. Do referable authorize the shaft to parch unimpeded of solvent. Run solvent from the shaft until the meniscus is impartial equalize with the culmination of the shaft.
    Use the pattern granted for your chromatography. Supplement this gently to the culmination of the shaft to a profundity of almost 1 cm, substance attentive referable to molest the gel. Turn the shaft on to authorize the meniscus to fall to the culmination of the Sephadex. Attentively supplement almost 1 cm3 of breathe-into to the culmination. Parch breathe-into from the deep, until the meniscus falls as distant as the culmination of the Sephadex. Repeat the supplementition and opposition of breathe-into. Repeat this order with 0.5M NaCl.
    Gently swell up the shaft with 0.5M NaCl and elute the breach at a tardy stream rebuke. Referablee the disconnection of the components as the shaft progresses.
    Collect the eluate in 10 cm3 portions and referablee the colour of each faction. Alternatively, students may test each faction spectroscopically. Cells of 1 cm method extension may be used although if the breachs are very pliant longer method extension cells may be essential.
    Once completed, elute the shaft with concentrated NaCl breach to displace any traces of issue. Empty the CM-Sephadex into the labelled collation beaker.

    raph Renn 21/08/ 170821 15271 Al(acac3 in CDCI coCl IC2280so2.-1 H NMR ppa 10 Al (acac in CDCs (3 ッ. NMR 20 20D

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