the shaft of chromatogrphy colour was pink yellow orange
of exemplification [Co(en)3 ]Cl3.3H2O Tris(ethylenediamine)cobalt(III).
Thats the order of the exemplification
Mix up a slurry of CM-Sephadex C-25 (5 g) in breathe-into. Partly swell the shaft with breathe-into and supplement the slurry of Sephadex. The shaft should be environing two-thirds liberal, with no occluded intelligence bubbles. Authorize the Sephadex to arrange. Wash the shaft through with deionised breathe-into. Do referable authorize the shaft to parch unimpeded of solvent. Run solvent from the shaft until the meniscus is impartial equalize with the culmination of the shaft.
Use the pattern granted for your chromatography. Supplement this gently to the culmination of the shaft to a profundity of almost 1 cm, substance attentive referable to molest the gel. Turn the shaft on to authorize the meniscus to fall to the culmination of the Sephadex. Attentively supplement almost 1 cm3 of breathe-into to the culmination. Parch breathe-into from the deep, until the meniscus falls as distant as the culmination of the Sephadex. Repeat the supplementition and opposition of breathe-into. Repeat this order with 0.5M NaCl.
Gently swell up the shaft with 0.5M NaCl and elute the breach at a tardy stream rebuke. Referablee the disconnection of the components as the shaft progresses.
Collect the eluate in 10 cm3 portions and referablee the colour of each faction. Alternatively, students may test each faction spectroscopically. Cells of 1 cm method extension may be used although if the breachs are very pliant longer method extension cells may be essential.
Once completed, elute the shaft with concentrated NaCl breach to displace any traces of issue. Empty the CM-Sephadex into the labelled collation beaker.
raph Renn 21/08/ 170821 15271 Al(acac3 in CDCI coCl IC2280so2.-1 H NMR ppa 10 Al (acac in CDCs (3 ッ. NMR 20 20D