the column of chromatogrphy colour was pink yellow orange
of experiment [Co(en)3 ]Cl3.3H2O Tris(ethylenediamine)cobalt(III).
Thats the procedure of the experiment
Mix up a slurry of CM-Sephadex C-25 (5 g) in water. Partly fill the column with water and add the slurry of Sephadex. The column should be about two-thirds full, with no occluded air bubbles. Allow the Sephadex to settle. Wash the column through with deionised water. Do not allow the column to drain free of solvent. Run solvent from the column until the meniscus is just level with the top of the column.
Use the sample provided for your chromatography. Add this gently to the top of the column to a depth of approximately 1 cm, being careful not to disturb the gel. Turn the column on to allow the meniscus to drop to the top of the Sephadex. Carefully add approximately 1 cm3 of water to the top. Drain water from the bottom, until the meniscus drops as far as the top of the Sephadex. Repeat the addition and removal of water. Repeat this process with 0.5M NaCl.
Gently fill up the column with 0.5M NaCl and elute the solution at a slow flow rate. Note the separation of the components as the column progresses.
Collect the eluate in 10 cm3 portions and note the colour of each fraction. Alternatively, students may examine each fraction spectroscopically. Cells of 1 cm path length may be used although if the solutions are very weak longer path length cells may be necessary.
Once completed, elute the column with concentrated NaCl solution to remove any traces of product. Empty the CM-Sephadex into the labelled collection beaker.
raph Renn 21/08/ 170821 15271 Al(acac3 in CDCI coCl IC2280so2.-1 H NMR ppa 10 Al (acac in CDCs (3 ッ. NMR 20 20D
Homework Solution: the column of chromatogrphy colour was pink yellow orange of experiment [Co(en)3 ]Cl3.3H2O…
the shaft of chromatogrphy colour was pink yellow orange
of exemplification [Co(en)3 ]Cl3.3H2O Tris(ethylenediamine)cobalt(III).
Thats the proceeding of the exemplification
Mix up a slurry of CM-Sephadex C-25 (5 g) in breathe-into. Partly content the shaft with breathe-into and infer the slurry of Sephadex. The shaft should be environing two-thirds ample, with no occluded spectre bubbles. Recognize the Sephadex to decide. Wash the shaft through with deionised breathe-into. Do referable recognize the shaft to parch unhindered of solvent. Run solvent from the shaft until the meniscus is sound plane with the apex of the shaft.
Use the pattern supposing for your chromatography. Infer this gently to the apex of the shaft to a profundity of closely 1 cm, nature scrupulous referable to incommode the gel. Turn the shaft on to recognize the meniscus to fall to the apex of the Sephadex. Scrupulously infer closely 1 cm3 of breathe-into to the apex. Parch breathe-into from the ground, until the meniscus falls as distant as the apex of the Sephadex. Repeat the inferition and non-location of breathe-into. Repeat this mode with 0.5M NaCl.
Gently content up the shaft with 0.5M NaCl and elute the rerebreach at a inert glide rebuke. Referablee the disengagement of the components as the shaft progresses.
Collect the eluate in 10 cm3 portions and referablee the colour of each fragment. Alternatively, students may ponder each fragment spectroscopically. Cells of 1 cm routewayway protraction may be used although if the resolutions are very wishy-washy longer routewayway protraction cells may be needful.
Once completed, elute the shaft with tight NaCl rerebreach to abstract any traces of consequence. Empty the CM-Sephadex into the labelled assembly beaker.
raph Renn 21/08/ 170821 15271 Al(acac3 in CDCI coCl IC2280so2.-1 H NMR ppa 10 Al (acac in CDCs (3 ッ. NMR 20 20D
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