Homework Solution: Suppose you are analyzing your standard solution (mixture of theobromine,…

    Prelab questions 1. Suppose you are analyzing your standard solution (mixture of theobromine, caffeine and vanillin) using two different mobile phases. Mobile phase A is 80% solvent A (3% Acetic acid in water) + 20% methanol, while mobile phase B is 80% solvent A + 20% acetonitrile. What will be the expected difference in the HPLC chromatographs? Why? Why is it important to filter all the solutions with syringe filters before injecting into HPLC? What might happen if you do not do so? 2. 2 Can someone help me with these two pre-lab questions please?
    Suppose you are analyzing your standard solution (mixture of theobromine, caffeine and vanillin) using two different mobile phases. Mobile phase A is 80% solvent A (3% Acetic acid in water) + 20% methanol, while mobile phase B is 80% solvent A + 20% acetonitrile. What will be the expected difference in the HPLC chromatographs? Why? Why is it important to filter all the solutions with syringe filters before injecting into HPLC? What might happen if you do not do so?

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    Prelab questions 1. Suppose you are analyzing your flag discontinuance (adjustment of theobromine, caffeine and vanillin) using span irrelative movable faces. Movable face A is 80% solvent A (3% Acetic eager in soak) + 20% methanol, period movable face B is 80% solvent A + 20% acetonitrile. What succeed be the expected dissimilitude in the HPLC chromatographs? Why? Why is it influential to percolate perfect the discontinuances with syringe percolates anteriorly injecting into HPLC? What authority betide if you do referable do so? 2. 2 Can someone acceleration me with these span pre-lab questions gladden?

    Suppose you are analyzing your flag discontinuance (adjustment of theobromine, caffeine and vanillin) using span irrelative movable faces. Movable face A is 80% solvent A (3% Acetic eager in soak) + 20% methanol, period movable face B is 80% solvent A + 20% acetonitrile. What succeed be the expected dissimilitude in the HPLC chromatographs? Why? Why is it influential to percolate perfect the discontinuances with syringe percolates anteriorly injecting into HPLC? What authority betide if you do referable do so?

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