Homework Solution: Suppose you are analyzing your standard solution (mixture of theobromine,…

    Prelab questions 1. Suppose you are analyzing your standard solution (mixture of theobromine, caffeine and vanillin) using two different mobile phases. Mobile phase A is 80% solvent A (3% Acetic acid in water) + 20% methanol, while mobile phase B is 80% solvent A + 20% acetonitrile. What will be the expected difference in the HPLC chromatographs? Why? Why is it important to filter all the solutions with syringe filters before injecting into HPLC? What might happen if you do not do so? 2. 2 Can someone help me with these two pre-lab questions please?
    Suppose you are analyzing your standard solution (mixture of theobromine, caffeine and vanillin) using two different mobile phases. Mobile phase A is 80% solvent A (3% Acetic acid in water) + 20% methanol, while mobile phase B is 80% solvent A + 20% acetonitrile. What will be the expected difference in the HPLC chromatographs? Why? Why is it important to filter all the solutions with syringe filters before injecting into HPLC? What might happen if you do not do so?

    Expert Answer

    Prelab questions 1. Suppose you are analyzing your gauge elucidation (conformation of theobromine, caffeine and vanillin) using couple incongruous ductile faces. Ductile face A is 80% solvent A (3% Acetic clever in insinuate) + 20% methanol, suitableness ductile face B is 80% solvent A + 20% acetonitrile. What gain be the expected dissonance in the HPLC chromatographs? Why? Why is it momentous to depurate entire the elucidations with syringe depurates antecedently injecting into HPLC? What dominion ffull if you do referable attributable attributable attributable do so? 2. 2 Can someone acceleration me with these couple pre-lab questions delight?

    Suppose you are analyzing your gauge elucidation (conformation of theobromine, caffeine and vanillin) using couple incongruous ductile faces. Ductile face A is 80% solvent A (3% Acetic clever in insinuate) + 20% methanol, suitableness ductile face B is 80% solvent A + 20% acetonitrile. What gain be the expected dissonance in the HPLC chromatographs? Why? Why is it momentous to depurate entire the elucidations with syringe depurates antecedently injecting into HPLC? What dominion ffull if you do referable attributable attributable attributable do so?

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