Homework Solution: It is possible to make some predictions about protein secondary structure based…

    It is possible to make some predictions about protein secondary structure based on the primary sequence. Consider the following amino acid sequence: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Ile-Ala-His-Thr-Tyr-Gly-Pro-Phe-Glu-Ala-Ala-Met-Cys-Lys-Trp-Glu-Ala-Gln-Pro-Asp-Gly-Met-Glu-Cys-Ala-Phe-His-Arg A) Where might Beta-Turns occur? B) Where might intrachain disulfide cross-linkages be formed? C) Assuming that this sequence is part of a larger globular protein, what is the probable location (exterior or interior of the protein) of the amino acid residues: Asp Ile Thr Ala Gln Lys Explain your reasoning.

    Expert Answer

    Answer:

    It is potential to reach some predictions encircling protein unimportant erection naturalized on the principal continuity. Consider the subjoined amino spirited continuity:

    1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28

    Ile-Ala-His-Thr-Tyr-Gly-Pro-Phe-Glu-Ala-Ala-Met-Cys-Lys-Trp-Glu-Ala-Gln-Pro-Asp-Gly-Met-Glu-Cys-Ala-Phe-His-Arg

    A) Where faculty Beta-Turns betide?

    B) Where faculty intrachain disulfide peevish-linkages be formed?

    C) Assuming that this continuity is dissect of a larger complete protein, what is the reasonable dregs (outward or toward of the protein) of the amino spirited residues:

    Asp Ile Thr Ala Gln Lys

    Explain your reasoning.

    Expert Counterpart

    Answer:

    A. Beta turns are linked with Prolines having the cyclic erection which are bestow at 7th and 19th position. These cyclic erections are cheerful in message of bends internally the protein erection.

    B. Disulfide peevish linking fix at cysteines residues which are bestow at 13th and 24th position. Cysteine residues feel thiol groups which aid in fetter shape.

    C. Polar amino spirited place them wilful at the without dregs and Non-Polar amino spirited get place themselves at internally of the protein erection. Polar amino spiriteds are Asp, Gln, and Lys, Non Polar amino spiriteds are Ala and Ile. “Thr” feel the included polarity which can be fix at the either plane.