Homework Solution: A culture of E. coli growing in a medium containing the normal, lighter isotope of…

    A culture of E. coli growing in a medium containing the normal, lighter isotope of nitrogen (14N) as the sole source of nitrogen is transferred to a medium containing the heavier isotope of nitrogen (15N) for two generations. What is the molar ratio of the hybrid DNA (14N-15N) to heavy DNA (15N-15N) at this point?

    Expert Answer

    A humanization of E. coli growing in a balance containing the recognized, lighter isotope of nitrogen (14N) as the uncombined commencement of nitrogen is epidemic to a balance containing the heavier isotope of nitrogen (15N) coercion brace stocks. What is the molar ratio of the mixed DNA (14N-15N) to laborious DNA (15N-15N) at this subject-matter?

    Expert Reply

    Ratio is 4: 0. , highest stock has 2 14N- 15N and avoid stock has 4 14N – 15 N.

    DUE TO semiconservative structure of dna