Homework Solution: A culture of E. coli growing in a medium containing the normal, lighter isotope of…

    A culture of E. coli growing in a medium containing the normal, lighter isotope of nitrogen (14N) as the sole source of nitrogen is transferred to a medium containing the heavier isotope of nitrogen (15N) for two generations. What is the molar ratio of the hybrid DNA (14N-15N) to heavy DNA (15N-15N) at this point?

    Expert Answer

    A culture of E. coli growing in a balance containing the usual, lighter isotope of nitrogen (14N) as the separate fountain of nitrogen is communicated to a balance containing the heavier isotope of nitrogen (15N) restraint span breeds. What is the molar appurtenancy of the impure DNA (14N-15N) to cumbrous DNA (15N-15N) at this object?

    Expert Counterpart

    Appurtenancy is 4: 0. , original breed has 2 14N- 15N and cooperate breed has 4 14N – 15 N.

    DUE TO semiconservative truth of dna